This study investigated the impact of competitive match play on Countermovement jump (CMJ) performance, hamstrings and adductor isometric strength, and subjective self-reported measures 40h post-competitive match play in professional soccer players. Twenty-eight pro-fessional players completed baseline assessments during pre-season. Assessment 40h post-match was limited to players who played ≥ 60 minutes, with players grouped into two cate-gories based on minutes played: 60–89 minutes or 90–110 minutes. Baseline measures for subjective self-reported measures were collected 24h before each match. Across both groups, significant reductions (p < 0.05) were observed in all outcome, ratio and driver metrics except for eccentric mean force. All strategy metrics significantly increased (p < 0.05). Minutes played and match demands were not significantly associated with CMJ performance (τᵇ = -0.07 - 0.16 and τᵇ = -0.28 - 0.00 respectively). Prone isometric 0 showed non-significant re-ductions (p > 0.05) in both conditions with trivial to small effects. Significant reductions (p < 0.05) were observed for both conditions for the supine 90 hamstring test, however minutes played, and match demands were not significantly associated with a reduction in perfor-mance (τᵇ = -0.04 - -0.01 and τᵇ = -0.27 - 0.21). Short lever hip adduction results showed non-significant reductions (p > 0.05) in the 60–89 minutes group, but significant decreases (p <.001) were observed for the dominant limb, non-dominant limb and total score in the 90–110 minutes condition. However, minutes played, and match demands were not significantly associated with reduction in adductor isometric strength (τᵇ = 0.18 - 0.22 and τᵇ = -0.15 - 0.11). Subjective measures revealed significant reductions in sleep quality (p < 0.05) and com-posite scores (p <.001), and significant increases in muscle soreness (p <.001) across both con-ditions. Mood was only significantly reduced (p < 0.05) in the 90-110 minutes condition. No significant associations were found between minutes played, match demand, and self-re-ported subjective scores (τᵇ = -0.07 - 0.05 and τᵇ = -0.09 – 0.00).