This study aimed to investigate the post-activation performance enhancement (PAPE) effects of “specific” (half back-squat) and “nonspecific” (bench press) conditioning activities on sprinting and jumping performances in female soccer players. Fourteen players (mean ± SD: age = 22.3 ± 4.0 years; body mass = 60.2 ± 7.8 kg; height = 164.1 ± 4.2 cm) competing at national level (1st League) participated in this within-subject crossover study. The players performed a warm-up protocol including 3 sets of 3 repetitions of half back-squat or bench press exercises at 90% 1RM, or a warm-up protocol without lifting weights (i.e., control condition). Forty-meter shuttle sprints (20 + 20 m with change of direction [COD-180°]), countermovement jump (CMJ), and horizontal jump (HJ) performances were recorded 6 min after the conditioning activities protocols or the control condition. Non-significant large positive effects were found for the HJ after the half back-squat (ES = 1.68; p > 0.05) and bench press (ES = 1.68; p > 0.05) protocols. Although non-significant, HJ changes (Δ = 0.07 m) were greater than the smallest worthwhile change (0.02 m) and standard error of measurement (0.03 m) following both conditioning activities. Moreover, no significant changes were found for sprint and CMJ performance after neither half back-squat nor bench press protocols (p > 0.05). In conclusion, both specific and nonspecific conditioning activities using heavy loads (i.e., 90% 1RM) may be suitable to enhance individual HJ. Finally, both conditioning activities are potentially ineffective for increasing sprint and CMJ performance in the context of this study.