The aim of the present study was to evaluate whether or not recreational soccer players (SP) and non-soccer players (non-SP) with similar intermittent-running ability had similar physiological responses to a soccer match-simulation protocol. Twenty-two recreational SP and 19 fitness-matched non-SP participated. Yo-Yo level 1 assessed intermittent-running ability, while the Loughborough Intermittent Shuttle Test served as soccer match-simulation protocol. Heart rate (HR), blood lactate concentration [La−] and rating of perceived exertion (RPE) were recorded after each bout (1–5, plus an exhaustive task). SP had lower HR after the third, fourth and fifth bout, compared to non-SP. Similarly, SP had lower [La−] after the third, fourth and the fifth bout. SP also had lower RPE after the third, fourth and fifth bout. The appropriateness of intermittent-running ability as the main determinant of physical performance in SP was questioned.