Uptake, biotransformation and elimination of selected pharmaceuticals in a freshwater invertebrate measured using liquid chromatography tandem mass spectrometry

Thomas, H, Miller, A, Bury, Nic, Owen, S.F and Barron, L.P (2017) Uptake, biotransformation and elimination of selected pharmaceuticals in a freshwater invertebrate measured using liquid chromatography tandem mass spectrometry. Chemosphere, 183. pp. 389-400. ISSN 0045-6535

[img]
Preview
Text
2017 Miller et al Chemosphere 183 389-400.pdf - Published Version

Download (1MB) | Preview

Abstract

Methods were developed to assess uptake and elimination kinetics in Gammarus pulex of nine pharmaceuticals (sulfamethazine, carbamazepine, diazepam, temazepam, trimethoprim, warfarin, metoprolol, nifedipine and propranolol) using targeted LC-MS/MS to determine bioconcentration factors (BCFs) using a 96 h toxicokinetic exposure and depuration period. The derived BCFs for these pharmaceuticals did not trigger any regulatory thresholds and ranged from 0 to 73 L kg−1 (sulfamethazine showed no bioconcentration). Metabolism of chemicals can affect accurate BCF determination through parameterisation of the kinetic models. The added selectivity of LC-MS/MS allowed us to develop confirmatory methods to monitor the biotransformation of propranolol, carbamazepine and diazepam in G. pulex. Varying concentrations of the biotransformed products; 4-hydroxypropranolol sulphate, carbamazepine-10,11-epoxide, nordiazepam, oxazepam and temazepam were measured following exposure of the precursor compounds. For diazepam, the biotransformation product nordiazepam was present at higher concentrations than the parent compound at 94 ng g−1 dw. Overall, the results indicate that pharmaceutical accumulation is low in these freshwater amphipods, which can potentially be explained by the rapid biotransformation and excretion.

Item Type: Article
Uncontrolled Keywords: Biotransformation, Pharmaceuticals, Gammarus pulex, Bioconcentration
Subjects: Q Science > QH Natural history
Divisions: Faculty of Health & Science > Department of Science & Technology
Depositing User: David Upson-Dale
Date Deposited: 22 Nov 2017 14:47
Last Modified: 22 Nov 2017 14:47
URI: http://oars.uos.ac.uk/id/eprint/273

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year