Hypoxia suppressed Copper toxicity during early development in Zebrafish embryos in a process mediated by the activation of the HIF signaling pathway.

Fitzgerald, J.A, Jameson, H.M, Fowler, V.H, Bond, G.L, Bickley, L.K, Webster, T.M, Bury, Nic, Wilson, R.J and Santos, E.M (2016) Hypoxia suppressed Copper toxicity during early development in Zebrafish embryos in a process mediated by the activation of the HIF signaling pathway. Environmental Science & Technology, 50 (8). pp. 4502-4512. ISSN 0013-936X

[img]
Preview
Text
2016 Fitzgerald et al EST.pdf - Published Version

Download (4MB) | Preview

Abstract

Hypoxia is a global and increasingly important stressor in aquatic ecosystems, with major impacts on biodiversity worldwide. Hypoxic waters are often contaminated with a wide range of chemicals but little is known about the interactions between these stressors. We investigated the effects of hypoxia on the responses of zebrafish (Danio rerio) embryos to copper, a widespread aquatic contaminant. We showed that during continuous exposures copper toxicity was reduced by over 2-fold under hypoxia compared to normoxia. When exposures were conducted during 24 h windows, hypoxia reduced copper toxicity during early development and increased its toxicity in hatched larvae. To investigate the role of the hypoxia signaling pathway on the suppression of copper toxicity during early development, we stabilized the hypoxia inducible factor (HIF) pathway under normoxia using a prolyl-4-hydroxylase inhibitor, dimethyloxalylglycine (DMOG) and demonstrated that HIF activation results in a strong reduction in copper toxicity. We also established that the reduction in copper toxicity during early development was independent of copper uptake, while after hatching, copper uptake was increased under hypoxia, corresponding to an increase in copper toxicity. These findings change our understanding of the current and future impacts of worldwide oxygen depletion on fish communities challenged by anthropogenic toxicants.

Item Type: Article
Uncontrolled Keywords: Hypoxia, Contamination, Toxicity, Zebrafish, Danio Rerio
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Health & Science > Department of Science & Technology
Depositing User: David Upson-Dale
Date Deposited: 27 Feb 2017 11:46
Last Modified: 20 Jun 2018 08:31
URI: http://oars.uos.ac.uk/id/eprint/187

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year