Osteogenic potential of Mesenchymal Stem Cells from Adipose Tissue, Bone Marrow and Hair Follicle Outer Root Sheath in a 3D Crosslinked Gelatin-Based Hydrogel

Li, Hanluo, Nawaz, Hafiz Awais, Masieri, Federica, Vogel, Sarah, Hempel, Ute, Bartella, Alexander K., Zimmerer, Rüdiger, Simon, Jan-Christoph, Schulz-Siegmund, Michaela, Hacker, Michael, Lethaus, Bernd and Savković, Vuk (2021) Osteogenic potential of Mesenchymal Stem Cells from Adipose Tissue, Bone Marrow and Hair Follicle Outer Root Sheath in a 3D Crosslinked Gelatin-Based Hydrogel. International Journal of Molecular Sciences, 22 (10). e5404. ISSN 1422-0067

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Abstract

Bone transplantation is regarded as the preferred therapy to treat a variety of bone defects. Autologous bone tissue is often lacking at the source, and the mesenchymal stem cells (MSCs) responsible for bone repair mechanisms are extracted by invasive procedures. This study explores the potential of autologous mesenchymal stem cells derived from the hair follicle outer root sheath (MSCORS). We demonstrated that MSCORS have a remarkable capacity to differentiate in vitro towards the osteogenic lineage. Indeed, when combined with a novel gelatin-based hydrogel called Osteogel, they provided additional osteoinductive cues in vitro that may pave the way for future application in bone regeneration. MSCORS were also compared to MSCs from adipose tissue (ADMSC) and bone marrow (BMMSC) in a 3D Osteogel model. We analyzed gel plasticity, cell phenotype, cell viability, and differentiation capacity towards the osteogenic lineage by measuring alkaline phosphatase (ALP) activity, calcium deposition, and specific gene expression. The novel injectable hydrogel filled an irregularly shaped lesion in a porcine wound model displaying high plasticity. MSCORS in Osteogel showed a higher osteo-commitment in terms of calcium deposition and expression dynamics of OCN, BMP2, and PPARG when compared to ADMSC and BMMSC, whilst displaying comparable cell viability and ALP activity. In conclusion, autologous MSCORS combined with our novel gelatin-based hydrogel displayed a high capacity for differentiation towards the osteogenic lineage and are acquired by non-invasive procedures, therefore qualifying as a suitable and expandable novel approach in the field of bone regeneration therapy.

Item Type: Article
Additional Information: ** From MDPI via Jisc Publications Router ** History: accepted 14-05-2021; pub-electronic 20-05-2021. ** Licence for this article: https://creativecommons.org/licenses/by/4.0/
Uncontrolled Keywords: hair follicle outer root sheath, mesenchymal stem cells, differentiation capacity towards osteogenic lineage, 3D injectable hydrogel, gelatin-based cross-linked hydrogel
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Health & Science > Department of Science & Technology
SWORD Depositor: Pub Router
Depositing User: Pub Router
Date Deposited: 27 May 2021 12:36
Last Modified: 27 May 2021 12:36
URI: http://oars.uos.ac.uk/id/eprint/1779

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