Heterologous expression of Ion Channels in Mammalian cell lines

Mathie, Alistair, Veale, Emma L and Holden, Robyn G (2021) Heterologous expression of Ion Channels in Mammalian cell lines. Methods in molecular biology, 2188. pp. 51-65. ISSN 1940-6029

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Abstract

Heterologous expression of recombinant ion channel subunits in mammalian cell lines allows for characterization of their functional properties and pharmacological regulation. In this chapter, we describe methods for thawing, refreezing, passaging, cell culture, and transfection of tsA201 cells suitable for electrophysiology and imaging experiments. Furthermore, we discuss the strengths and limitations of using these methods.

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Health & Science > Department of Science & Technology
Depositing User: Alistair Mathie
Date Deposited: 01 Mar 2021 10:55
Last Modified: 01 Mar 2021 10:55
URI: http://oars.uos.ac.uk/id/eprint/1652

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