Time- and dose-dependent effects of chronic wound fluid on human adult dermal fibroblasts.

De Mattei, Monica, Ongaro, Alessia, Magaldi, Simona, Gemmati, Donato, Legnaro, Andrea, Palazzo, Annunziata, Masieri, Federica, Pellati, Agnese, Catozzi, Linda, Caruso, Angelo and Zamboni, Paolo (2008) Time- and dose-dependent effects of chronic wound fluid on human adult dermal fibroblasts. Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 34 (3). pp. 347-56. ISSN 1524-4725

Full text not available from this repository.



Wound healing is a biologic process that is altered in patients affected by chronic venous ulcers. The wound microenvironment is reflected in the chronic wound fluid (CWF), an exudate containing serum components and tissue-derived proteins.


We investigated the effects of increasing doses of CWF collected from patients suffering from chronic venous ulcers on human adult dermal fibroblasts cultured in vitro and the relationship among CWF effects and treatment length.


Fibroblasts were treated with 60, 240, and 720 microg/mL CWF for 3 and 7 days. We evaluated cell proliferation and viability by MTT and Trypan blue assay, cell morphology by light microscopy, F-actin microfilaments organization by tetramethylrhodamine B isothiocyanate-conjugated phalloidin, alpha-smooth muscle actin expression by immunofluorescence, and senescence-associated beta-galactosidase activity.


CWF induced an increase in cell proliferation in the first 3 days of treatment. In contrast, at 7 days, a strong decrease in cell viability was observed. These changes were related to a cytoskeletal F-actin reorganization and not to fibroblast-myofibroblast differentiation nor to changes in cellular senescence.


This study shows a dose-dependent and biphasic effect of CWF on dermal fibroblasts, suggesting that a continuous exposure to chronic wounds microenvironment may induce late cellular dysfunctions possibly involved in the delayed wound healing.

Item Type: Article
Uncontrolled Keywords: wound healing, wound fluid, adult dermal fibroblasts
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
R Medicine > RL Dermatology
Divisions: Faculty of Health & Science > Department of Science & Technology
Depositing User: Federica Masieri
Date Deposited: 23 Feb 2021 09:29
Last Modified: 01 Mar 2021 16:09
URI: https://oars.uos.ac.uk/id/eprint/1633

Actions (login required)

View Item View Item