The middle part of the plucked hair follicle outer root sheath is identified as an area rich in lineage-specific stem cell markers

Li, Hanluo, Masieri, Federica, Schneider, Marie, Bartella, Alexander, Gaus, Sebastian, Hahnel, Sebastian, Zimmerer, Rüdiger, Sack, Ulrich, Maksimovic-Ivanic, Danijela, Mijatovic, Sanja, Simon, Jan-Christoph, Lethaus, Bernd and Savkovic, Vuk (2021) The middle part of the plucked hair follicle outer root sheath is identified as an area rich in lineage-specific stem cell markers. Biomolecules, 11 (2). e154. ISSN 2218-273X

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Abstract

Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.

Item Type: Article
Uncontrolled Keywords: hair follicle, outer root sheath, mesenchymal stem cells, neuroectodermal cells, non-invasive sampling, multipotency, in vitro cultivation
Subjects: Q Science > QM Human anatomy
Divisions: Faculty of Health & Science > Department of Science & Technology
SWORD Depositor: Pub Router
Depositing User: Pub Router
Date Deposited: 08 Feb 2021 09:43
Last Modified: 08 Feb 2021 09:43
URI: http://oars.uos.ac.uk/id/eprint/1592

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