Autologous, non-invasively available mesenchymal stem cells from the outer root sheath of hair follicle are obtainable by migration from plucked hair follicles and expandable in scalable amounts

Li, Hanluo, Masieri, Federica, Schneider, Marie, Kottek, Tina, Hahnel, Sebastian, Yamauchi, Kensuke, Obradović, Danilo, Seon, Jong-Keun, Yun, Sook Jung, Ferrer, Rubén A., Franz, Sandra, Simon, Jan-Christoph, Lethaus, Bernd and Savković, Vuk (2020) Autologous, non-invasively available mesenchymal stem cells from the outer root sheath of hair follicle are obtainable by migration from plucked hair follicles and expandable in scalable amounts. Cells, 9 (9). e2069. ISSN 2073-4409

[img]
Preview
Text
cells-09-02069-v2.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (3MB) | Preview

Abstract

Background: Regenerative therapies based on autologous mesenchymal stem cells (MSC) as well as stem cells in general are still facing an unmet need for non-invasive sampling, availability, and scalability. The only known adult source of autologous MSCs permanently available with no pain, discomfort, or infection risk is the outer root sheath of the hair follicle (ORS). Methods: This study presents a non-invasively-based method for isolating and expanding MSCs from the ORS (MSCORS) by means of cell migration and expansion in air−liquid culture. Results: The method yielded 5 million cells of pure MSCORS cultured in 35 days, thereby superseding prior art methods of culturing MSCs from hair follicles. MSCORS features corresponded to the International Society for Cell Therapy characterization panel for MSCs: adherence to plastic, proliferation, colony forming, expression of MSC-markers, and adipo-, osteo-, and chondro-differentiation capacity. Additionally, MSCORS displayed facilitated random-oriented migration and high proliferation, pronounced marker expression, extended endothelial and smooth muscle differentiation capacity, as well as a paracrine immunomodulatory effect on monocytes. MSCORS matched or even exceeded control adipose-derived MSCs in most of the assessed qualities. Conclusions: MSCORS qualify for a variety of autologous regenerative treatments of chronic disorders and prophylactic cryopreservation for purposes of acute treatments in personalized medicine.

Item Type: Article
Uncontrolled Keywords: mesenchymal stem cells, hair follicle, regenerative medicine, non-invasive, cell therapy, adipose tissue, differentiation
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Health & Science > Department of Science & Technology
SWORD Depositor: Pub Router
Depositing User: Pub Router
Date Deposited: 17 Sep 2020 09:37
Last Modified: 17 Sep 2020 09:37
URI: http://oars.uos.ac.uk/id/eprint/1389

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year