Hatching gland development and hatching in zebrafish embryos: A role for zinc and its transporters Zip10 and Znt1a

Muraina, Issa Atanda, Maret, Wolfgang, Bury, Nic, Scott, Annabella, Graham, Anthony and Hogstrand, Christer (2020) Hatching gland development and hatching in zebrafish embryos: A role for zinc and its transporters Zip10 and Znt1a. Biochemical and Biophysical Research Communications. ISSN 0006-291X (In Press)

[img] Text
BBRC-S-20-05698_FINAL2.pdf - Accepted Version
Restricted to Repository staff only until 31 December 2021.
Available under License Creative Commons Attribution Non-commercial Share Alike.

Download (1MB) | Request a copy

Abstract

Zinc transporters of the Zip (Slc39, importers) and ZnT (Slc30, exporters) protein families have evolutionary conserved roles in biology. The aim of the present study was to explore the role of zinc, and zinc transporters Zip10 and Znt1a in zebrafish hatching gland development and larval hatching. In the study, knockdown of genes for Zip10 and Znt1a in zebrafish embryos was achieved using morpholino-modified oligonucleotides. A partial loss-of-function Znt1a mutant (Znt1asa17) allowed comparison with the Znt1a morphant. Free Zn2+ in embryos and apoptosis were investigated using fluorescent dyes whereas gene expression was investigated by whole-mount insitu hybridization. The results showed high levels of free Zn2+ in the hatching gland cells(HGC) along with abundant expression of zip10 and znt1a in normal embryo. Knockdown of zip10 reduced free Zn2+ In HGC,ceased their normal developmental apoptosis,and resulted indisplacement and later disappearance of hatching glands and hatching enzymes he1a and catL1b, and inability to hatch. Conversely, knockdown of znt1a or the Znt1 as a 17 mutation accelerated hatching and coincided with high expression of hatching enzymes and free Zn2+ in the HGC. Thus, Zip10 and free Zn2+ in the HGC are required both for their development and function.This study also demonstrated the opposite functions of the two zinc transporters, ZIP10 and ZnT1 as well as shedding light on the role of Zn2+ in regulation of the human hatching enzyme homologue, ovastacin, which is activated by zinc and cleaves the zona pellucida protein, ZP2, to prevent polyspermy.

Item Type: Article
Uncontrolled Keywords: Zebrafish, Slc30a1, Slc39a10, apoptosis, Zn, he1a, catL1b
Subjects: Q Science > QL Zoology
Divisions: Faculty of Health & Science > Department of Science & Technology
Depositing User: Nic Bury
Date Deposited: 28 May 2020 10:18
Last Modified: 28 May 2020 10:18
URI: http://oars.uos.ac.uk/id/eprint/1250

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year